Article
Structural basis for RNA recognition by NusB and NusE in the initiation of transcription antitermination.
Nucleic acids research - 1 Sept 2011
Stagno Jason R, Altieri Amanda S, Bubunenko Mikhail, Tarasov Sergey G, Li Jess, Court Donald L, Byrd R Andrew, Ji Xinhua
Abstract excerpt
Processive transcription antitermination requires the assembly of the complete antitermination complex, which is initiated by the formation of the ternary NusB-NusE-BoxA RNA complex. We have elucidated the crystal structure of this complex, demonstrating that the BoxA RNA is composed of 8 nt that are recognized by the NusB-NusE heterodimer. Functional biologic and biophysical data support the structural...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Binding Sites
- Escherichia coli Proteins
- Genetic Complementation Test
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Phenotype
- Protein Multimerization
- RNA
- RNA, Double-Stranded
- RNA-Binding Proteins