Novel application of 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole to identify cysteine sulfenic acid in the AhpC component of alkyl hydroperoxide reductase.

doi:10.1021/bi972191x

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Article

Novel application of 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole to identify cysteine sulfenic acid in the AhpC component of alkyl hydroperoxide reductase.

Biochemistry - 2 Dec 1997

Ellis H R, Poole L B

Abstract excerpt

The trapping of a sulfenic acid within the fully active C165S mutant of the AhpC peroxidase protein from Salmonella typhimurium was investigated. The electrophilic reagent employed in these studies, 7-chloro-4-nitrobenz-2-oxa-1,3-diazole (NBD-Cl), has previously been used to modify thiol, amino,...

Topics

4-Chloro-7-nitrobenzofurazan
Cyclohexanones
Cysteine
Mass Spectrometry
Mutation
Oxidoreductases
Peroxidases
Peroxiredoxins
Salmonella typhimurium
Spectrophotometry
Sulfenic Acids
Sulfhydryl Compounds

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