Major increase in endopeptidase activity of human cathepsin B upon removal of occluding loop contacts.

doi:10.1021/bi971264+

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Article

Major increase in endopeptidase activity of human cathepsin B upon removal of occluding loop contacts.

Biochemistry - 14 Oct 1997

Nägler D K, Storer A C, Portaro F C, Carmona E, Juliano L, Ménard R

Abstract excerpt

The main feature distinguishing cathepsin B from other cysteine proteases of the papain family is the presence of a large insertion loop, termed the occluding loop, which occupies the S' subsites of the enzyme. The loop is held in place mainly by two contacts with the rest of the enzyme, involvin...

Topics

Cathepsin B
Endopeptidases
Humans
Hydrogen-Ion Concentration
Hydrolysis
Mutation
Protein Conformation

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