Primary structure requirements for in vivo activity and bidirectional function of the transcription terminator shared by the oppositely oriented skc/rel-orf1 genes of Streptococcus equisimilis H46A.

Abstract excerpt

The region between the Streptococcus equisimilis streptokinase (skc) gene and the oppositely oriented rel-orf1 transcription unit contains only one termination site known to function bidirectionally in both the homologous host and in Escherichia coli. The terminator sequence is similar to other factor-independent terminators. Using two sets of point mutations that interrupt the hairpin-upstream oligo(dA) tract or...

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