Article
A human monoclonal anti-D antibody which detects a nonconformation-dependent epitope on the RhD protein by immunoblot.
British journal of haematology - 1 Aug 1997
Apoil P A, Reid M E, Halverson G, Mouro I, Colin Y, Roubinet F, Cartron J P, Blancher A
Abstract excerpt
We describe the first human monoclonal anti-D (LOR-15C9) which reacts with a D-specific motif exposed either on a native form on intact D-positive red cells or on a denatured form of the RhD protein (33 kD), and detected by immunoblotting. LOR-15C9 was able to precipitate RhD but not RhcE proteins produced by in vitro transcription-translation assays. The reactivity of the antibody, using panels of red cells with...
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