Article
Variations in culture pH affect the cloning efficiency and differentiation of progenitor cells in <i>ex vivo</i> haemopoiesis
1 Jun 1997
Abstract excerpt
Haemopoietic cultures may experience pH variations of as much as 0.5 units depending on culture duration and cell density. Since pH is a potent modulator of cellular proliferation and differentiation, we examined its effects on the performance of both semisolid and liquid haemopoietic cultures. Culture pH was found to have substantial effects both on progenitor cloning efficiency (as measured in liquid cultures)...
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