Article
Analysis of the Legionella pneumophila fliI gene: intracellular growth of a defined mutant defective for flagellum biosynthesis.
Infection and immunity - 1 Jun 1997
Merriam J J, Mathur R, Maxfield-Boumil R, Isberg R R
Abstract excerpt
Using a PCR-based strategy and degenerate oligonucleotides, we isolated a Legionella pneumophila gene that showed high sequence similarity to members of the fliI gene family. An insertion mutation that disrupted the fliI open reading frame was recombined onto the L. pneumophila chromosome and ana...
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