Scanning method to identify the molecular heterogeneity of delta-globin gene especially in delta-thalassemias: detection of three novel substitutions in the promoter region of the gene.

doi:10.1002/(SICI)1098-1004(1997)9:5<465::AID-HUMU14>3.0.CO;2-0

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Article

Scanning method to identify the molecular heterogeneity of delta-globin gene especially in delta-thalassemias: detection of three novel substitutions in the promoter region of the gene.

Human mutation - 1 Jan 1997

Papadakis M, Papapanagiotou E, Loutradi-Anagnostou A

Abstract excerpt

A scanning strategy for the detection of delta-globin gene mutations and polymorphisms is presented. This procedure is based on the denaturing gradient gel electrophoresis (DGGE) of four different artificially amplified DNA fragments which cover the promoter, the exons, as well as IVS I of the re...

Topics

Electrophoresis, Polyacrylamide Gel
Genetic Carrier Screening
Genetic Heterogeneity
Globins
Humans
Mutation
Promoter Regions, Genetic
Thalassemia

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