Article
Rapid identification by denaturing gradient gel electrophoresis of mutations in the gamma-globin gene promoters in non-deletion type HPFH.
British journal of haematology - 1 Apr 1992
Gottardi E, Losekoot M, Fodde R, Saglio G, Camaschella C, Bernini L F
Abstract excerpt
We have outlined a fast, non-radioactive strategy to identify point mutations in the 5' flanking region of the gamma-globin genes using denaturing gradient gel electrophoresis (DGGE) of amplified DNA. In a sample of previously characterized carriers of non deletion-type hereditary persistence of fetal haemoglobin (HPFH) the different point mutations in both gamma gene promoters could be easily identified by DGGE...
Topics
- DNA Mutational Analysis
- Electrophoresis, Polyacrylamide Gel
- Fetal Hemoglobin
- Globins
- Hemoglobinopathies
- Humans
- Mutation
- Polymerase Chain Reaction
- Promoter Regions, Genetic
- Protein Denaturation