Article
Analysis of influenza A H3N2 strains isolated in England during 1995-1996 using polymerase chain reaction restriction.
Journal of medical virology - 1 Mar 1997
Ellis J S, Sadler C J, Laidler P, de Andrade H R, Zambon M C
Abstract excerpt
A polymerase chain reaction-restriction (PCR-restriction) endonuclease assay was developed to allow rapid analysis of influenza A H3N2 viruses circulating in England during 1995-1996. Restriction endonuclease digestion with two enzymes of amplicons derived from PCR of the HA1 portion of the influenza haemagglutinin (HA) gene was able to differentiate antigenically similar influenza strains into two groups. Group...
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