Article
Insertionally inactivated and inducible recA alleles for use in Neisseria.
Gene - 1 Apr 1997
Seifert H S
Abstract excerpt
Two classes of recA mutations have been constructed for use in Neisseria gonorrhoeae: three insertionally inactivated ('knockout') mutations and three LacI-regulatable constructs that can be shifted between Rec- and Rec+ by the removal or addition of IPTG. The effects of regulating recA expression on the processes of DNA transformation, DNA repair and pilin-phase variation are described. These regulatable...
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