Article
Mechanism-based inactivation of cytochrome P450 2B4 by aldehydes: relationship to aldehyde deformylation via a peroxyhemiacetal intermediate.
Biochemistry - 22 Apr 1997
Raner G M, Chiang E W, Vaz A D, Coon M J
Abstract excerpt
The inactivation of cytochrome P450 2B4 by aldehydes in a reconstituted enzyme system requires molecular oxygen and NADPH and is not prevented by the addition of catalase, superoxide dismutase, epoxide hydrolase, glutathione, or ascorbic acid. A strong correlation between loss of enzymatic activi...
Topics
- Aldehydes
- Aryl Hydrocarbon Hydroxylases
- Ascorbic Acid
- Catalase
- Chromatography, High Pressure Liquid
- Cytochrome P-450 Enzyme Inhibitors
- Cytochrome P-450 Enzyme System
- Deuterium
- Enzyme Inhibitors
- Epoxide Hydrolases
- Free Radicals
- Glutathione
- Models, Chemical
- Mutation
- NADP
- Steroid Hydroxylases
- Superoxide Dismutase