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Article

Further Development and Use of a Molecular Serotype Identification Test for Infectious Bronchitis Virus

1 Jan 1997

Abstract excerpt

Previously, we developed a rapid serotype identification test for infectious bronchitis virus (IBV) that utilizes the reverse transcriptase-polymerase chain reaction (RT-PCR) and restriction fragment length polymorphism analysis. The RT-PCR is used to amplify the S1 gene from RNA extracted from the virus grown in eggs. Restriction enzyme digestion and electrophoresis of that PCR product is used to determine the...

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PMID: 9087326
DOI: 10.2307/1592449

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