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Differentiation of Infectious Bronchitis Virus Serotypes Using Polymerase Chain Reaction and Restriction Fragment Length Polymorphism Analysis

1 Jan 1993

Abstract excerpt

Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis were used to differentiate between serotypes of several infectious bronchitis virus (IBV) strains. A sequence of 1720 base pairs (bp) that contains the S1 glycoprotein gene of IBV was amplified by PCR, purified, and digested with restriction enzymes. Eleven reference IBV strains were grouped according to the RFLP...

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PMID: 8095782
DOI: 10.2307/1591474

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