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Article

Rapid detection and identification of avian infectious bronchitis virus.

Journal of clinical microbiology - 1 Jan 1992

Zwaagstra K A, van der Zeijst B A, Kusters J G

Abstract excerpt

A rapid and sensitive method for the detection and unambiguous typing of infectious bronchitis virus (IBV) is described. RNA was isolated from IBV-infected allantoic fluid and was transcribed into cDNA. This cDNA was amplified by the polymerase chain reaction. The polymerase chain reaction products were subsequently analyzed on an agarose gel. The presence of IBV-specific RNA in the allantoic fluid then allowed...

Topics

Animals
Base Sequence
DNA Probes
DNA, Viral
Genetic Variation
Infectious bronchitis virus
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction
Poultry
RNA, Viral

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 1310335
DOI: 10.1128/jcm.30.1.79-84.1992
PMCID: PMC265000

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1310335-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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