Article
A multiplex reverse transcriptase-PCR assay for the genotyping of avian infectious bronchitis viruses.
Avian diseases - 1 Mar 2010
Chen Hui-Wen, Wang Ching-Ho
Abstract excerpt
Infectious bronchitis viruses (IBVs) in Taiwan have been divided into two genogroups, Taiwan group I (TW-I) and Taiwan group II (TW-II). Heterologous Mass-type strains are widely used as vaccines in the field. This work reports on a rapid and reliable multiplex reverse transcriptase-polymerase chain reaction (mRT-PCR) assay for the genotyping of IBVs. Multiplex primer sets were designed to amplify the region...
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