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Identification of Avian Infectious Bronchitis Virus by Direct Automated Cycle Sequencing of the S-1 Gene

1 Apr 2000

Abstract excerpt

Direct automated cycle sequencing (DACS) of a reverse transcription-polymerase chain reaction (RT-PCR) product of the S-1 subunit of the spike peplomer gene was used to identify infectious bronchitis virus (IBV) serotypes. Degenerate primers CK4 and CK2, utilized previously in our laboratory, were selected for DACS because they successfully amplify a wide range of serotypes represented by various reference...

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PMID: 10879913
DOI: 10.2307/1592547

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