Article
A genetic strategy for differential screening of meiotic germ-cell cDNA libraries.
Molecular reproduction and development - 1 Apr 1996
Caldwell K A, Wiltshire T, Handel M A
Abstract excerpt
The goals of this work were to create germ-cell-stage-specific cDNA libraries from mouse spermatogenic cells and to employ a novel two-step genetic screen to identify gene sequences present during the critical meiotic stage of spermatogenesis. Highly enriched germ-cell fractions were prepared from adult and juvenile mouse testes, and purity of these fractions was extensively analyzed by light and electron...
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