Article
Analysis of HLA-DRB1 alleles using PCR-RFLP and PCR-MPH.
The Southeast Asian journal of tropical medicine and public health - 1 Mar 1996
Thalang O N, Sukha C, Suwanasophon C, Maneemaroj R, Okubo Y, Tatsumi N
Abstract excerpt
In this study we compare the results of HLA-DRB1 genotyping by PCR-RFLP and PCR-MPH. HLA-DR specificities were also performed by LCT. Samples were obtained from 20 Thai patients who were on the waiting list for kidney transplant. DNA was extracted by phenol-chloroform extraction. It was found that the results gave complete agreement with two methods of DNA typing, however, there were 3 discrepancies in assigning...
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