Role of His243 in the phosphatase activity of EnvZ in Escherichia coli.

doi:10.1128/jb.179.4.1413-1416.1997

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Article

Role of His243 in the phosphatase activity of EnvZ in Escherichia coli.

Journal of bacteriology - 1 Feb 1997

Skarphol K, Waukau J, Forst S A

Abstract excerpt

EnvZ undergoes autophosphorylation at His243 and subsequently transfers the phosphate group to OmpR. EnvZ also possesses an OmpR-phosphate phosphatase activity. We examined the role of His243 in the phosphatase function by replacing His with either Val, Tyr, Ser, Asp, or Asn. EnvZH243V and EnvZH2...

Topics

Bacterial Outer Membrane Proteins
Escherichia coli
Escherichia coli Proteins
Histidine
Multienzyme Complexes
Mutagenesis
Mutation
Phosphoprotein Phosphatases
Phosphorylation

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