Article
Isolation of Highly Purified Type A Spermatogonia From Prepubertal Rat Testis
12 Nov 1996
Abstract excerpt
We have developed a new method that allows isolation of highly purified type A spermatogonia from prepubertal rats. The procedure is based on the maximal release of spermatogonia from the seminiferous epithelium obtained by the complete enzymatic digestion of the tubular basal lamina, followed by removal of contaminating somatic cells through adhesion to plastic dishes coated with the lectin Datura stramonium...
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