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Article

Proliferation and Differentiation of Bovine Type A Spermatogonia During Long-Term Culture1

1 Jan 2003

Abstract excerpt

The present study was aimed at developing a method for long-term culture of bovine type A spermatogonia. Testes from 5-mo-old calves were used, and pure populations of type A spermatogonia were isolated. Cells were cultured in minimal essential medium (MEM) or KSOM (potassium-rich medium prepared according to the simplex optimization method) and different concentrations of fetal calf serum (FCS) for 2-4 wk at 32...

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PMID: 12493723
DOI: 10.1095/biolreprod.102.004986

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