Article
Internal and flanking sequence from AFLP fragments using ligation-mediated suppression PCR.
BioTechniques - 1 May 1999
Schupp J M, Price L B, Klevytska A, Keim P
Abstract excerpt
Amplification fragment-length polymorphism (AFLP) analysis has proven to be a powerful tool for developing a large number of reliable genetic markers across a wide variety of organisms. Often it is desirable to further characterize these markers by obtaining internal and flanking sequence information. Here, we present a systematic approach for obtaining such information from AFLP markers. AFLP fragments can be...
Topics
- Alleles
- Bacillus anthracis
- Base Sequence
- Biotechnology
- DNA Primers
- DNA, Bacterial
- Gene Amplification
- Genetic Markers
- Polymerase Chain Reaction
- Polymorphism, Genetic
- Sequence Alignment
