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Development of a versatile, target-oriented tiling microarray assay for measuring allele-specific gene expression.

Genomics - 1 Nov 2010

He Hang, Zhang Huiyong, Wang Xiangfeng, Wu Nicholas, Yang Xiaozeng, Chen Runsheng, Li Yi, Deng Xing Wang, Li Lei

Abstract excerpt

In the study of gene expression, it is often desirable to distinguish transcript pools derived from different alleles present in the same organism. We report here an oligonucleotide tiling microarray designed to specifically target 518 single nucleotide polymorphisms (SNPs) between the two sequenced rice (Oryza sativa) subspecies indica and japonica. The tiling array included all 25-mer probes interrogating each...

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PMID: 20688151
DOI: 10.1016/j.ygeno.2010.07.008

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