PCR/RFLP assay for copy number of mutant and wild-type alleles.

Abstract excerpt

A PCR method for quantitating the copy number of mutant vs. wild-type alleles in DNA from cell lines is described. The assay can be used to detect a point mutation in any gene that creates or destroys a restriction site. The alleles of interest are amplified by nested PCR and labeled in a second round of PCR. The product is digested with a restriction enzyme specific for that site, resolved on a non-denaturing...

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• Share to Polymorphism, Restriction Fragment Length
• Share to Alleles
• Share to DNA, Neoplasm