Article
Reversal of the in vivo metastatic phenotype of human tumor cells by an anti-CAPL (mts1) ribozyme.
Cancer research - 1 Dec 1996
Maelandsmo G M, Hovig E, Skrede M, Engebraaten O, Flørenes V A, Myklebost O, Grigorian M, Lukanidin E, Scanlon K J, Fodstad O
Abstract excerpt
The putative role of the CAPL gene in enhancing the development of human cancer metastasis was examined by transfecting human high-expressing osteosarcoma cells with a hammerhead ribozyme directed against the gene transcript. The ability of the ribozyme to cleave target mRNA in intact cells was demonstrated in a 5'-rapid amplification of cDNA ends assay. In transfected cells, a suppression of the capacity to give...
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