Article
A PCR-based method of detection and differentiation of K88+ adhesive Escherichia coli.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc - 1 Oct 1996
Franklin M A, Francis D H, Baker D, Mathew A G
Abstract excerpt
The objective of this study was to develop a polymerase chain reaction (PCR)-based method to detect and differentiate among Escherichia coli strains containing genes for the expression of 3 antigenic variants of the fimbrial adhesin K88 (K88ab, K88ac, and K88ad). Five primers were designed that a...
Topics
- Adhesins, Escherichia coli
- Animals
- Bacterial Adhesion
- Blotting, Western
- DNA Primers
- Diarrhea
- Enzyme-Linked Immunosorbent Assay
- Escherichia coli
- Escherichia coli Infections
- Genetic Variation
- Plasmids
- Polymerase Chain Reaction
- Swine
- Swine Diseases