Article
Analysis of the F antigen-specific papA alleles of extraintestinal pathogenic Escherichia coli using a novel multiplex PCR-based assay.
Infection and immunity - 1 Mar 2000
Johnson J R, Stell A L, Scheutz F, O'Bryan T T, Russo T A, Carlino U B, Fasching C, Kavle J, Van Dijk L, Gaastra W
Abstract excerpt
Polymorphisms in PapA, the major structural subunit and antigenic determinant of P fimbriae of extraintestinal pathogenic Escherichia coli, are of considerable epidemiological, phylogenetic, and immunotherapeutic importance. However, to date, no method other than DNA sequencing has been generally available for their detection. In the present study, we developed and rigorously validated a novel PCR-based assay for...
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