Article
Differentiation of bacterial 16S rRNA genes and intergenic regions and Mycobacterium tuberculosis katG genes by structure-specific endonuclease cleavage.
Journal of clinical microbiology - 1 Dec 1996
Brow M A, Oldenburg M C, Lyamichev V, Heisler L M, Lyamicheva N, Hall J G, Eagan N J, Olive D M, Smith L M, Fors L, Dahlberg J E
Abstract excerpt
We describe here a new approach for analyzing nucleic acid sequences using a structure-specific endonuclease, Cleavase I. We have applied this technique to the detection and localization of mutations associated with isoniazid resistance in Mycobacterium tuberculosis and for differentiating bacterial genera, species and strains. The technique described here is based on the observation that single strands of DNAs...
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