Article
Bacterial production of transdihydroxycyclohexadiene carboxylates by metabolic pathway engineering.
Microbiology (Reading, England) - 1 Apr 1996
Müller Rolf, Breuer Michael, Wagener Andreas, Schmidt Karsten, Leistner Eckhard
Abstract excerpt
Homochiral-cis-cyclohexa-3,5-diene-1,2-diols are important synthons. We found a way to produce trans-configured homochiral diols using recombinant Klebsiella pneumoniae 62-1. Transformation of this mutant (Phe- Trp- Tyr-) with plasmids carrying genes involved in chorismic and isochorismic acid me...
Topics
- Amino Acid Sequence
- Base Sequence
- Carboxylic Acids
- Chorismic Acid
- Cyclohexanols
- Cyclohexenes
- DNA Primers
- DNA, Bacterial
- Escherichia coli
- Genes, Bacterial
- Genetic Engineering
- Hydrolases
- Intramolecular Transferases
- Iron
- Isomerases
- Klebsiella pneumoniae
- Molecular Sequence Data
- Mutation