Article
Glycosylated human growth hormone (hGH): a novel 24 kDa hGH-N variant.
Biochemical and biophysical research communications - 12 Nov 1996
Haro L S, Lewis U J, Garcia M, Bustamante J, Martinez A O, Ling N C
Abstract excerpt
We have identified a human pituitary protein as a novel glycosylated variant of hGH. Isolation of the denatured protein included separation of human pituitary extract by Sephadex G-100 chromatography in ammonium bicarbonate, followed by Sephadex G-100 chromatography in 10% acetic acid, with subsequent DEAE Sephacryl chromatography in ammonium bicarbonate, and finally by preparative SDS PAGE. The pituitary protein...
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