Article
N-glycosylated variants of growth hormone in human pituitary extracts.
Hormone research - 1 Jan 2000
García-Barros M, Costoya J A, Ríos R, Arce V, Devesa J
Abstract excerpt
This study was designed to investigate the existence, in human pituitary extracts, of growth hormone (GH) variants not encoded by the hGH-N gene. Using anion exchange-fast protein liquid chromatography followed by SDS-PAGE, we isolated several basic forms of pituitary GH. Incubation of these basic forms with endoglycosidase F/N-glycosidase F revealed that two of them (about 34 and 12 kD) were N-glycosylated. In...
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