An Arabidopsis mutant deficient in sterol biosynthesis: heterologous complementation by ERG 3 encoding a delta 7-sterol-C-5-desaturase from yeast.

doi:10.1046/j.1365-313x.1995.08030407.x

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Article

An Arabidopsis mutant deficient in sterol biosynthesis: heterologous complementation by ERG 3 encoding a delta 7-sterol-C-5-desaturase from yeast.

The Plant journal : for cell and molecular biology - 1 Sept 1995

Gachotte D, Meens R, Benveniste P

Abstract excerpt

The mutant STE 1 was isolated by screening an ethylmethane sulfonate (EMS)-mutagenized population of Arabidopsis thaliana which consisted of 22,000 M2 plants divided into 1100 pools of 20 plants by gas chromatography of sterols extracted from small leaf samples. STE 1 was characterized by the accumulation of three delta 7-sterols concomitantly with the decrease of the three corresponding delta 5-sterols which are...

Topics

Arabidopsis
Base Sequence
Chromatography, High Pressure Liquid
Crosses, Genetic
Gas Chromatography-Mass Spectrometry
Genes, Fungal
Genetic Complementation Test
Molecular Sequence Data
Mutagenesis, Site-Directed
Mutation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Oxidoreductases

Plant Roots

Plants, Genetically Modified

Polymerase Chain Reaction

Saccharomyces cerevisiae

Sterols

Source

Open in PubMed

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PMID: 7550378
DOI: 10.1046/j.1365-313x.1995.08030407.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7550378-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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An Arabidopsis mutant deficient in sterol biosynthesis: heterologous complementation by ERG 3 encoding a delta 7-sterol-C-5-desaturase from yeast.PMID 7550378Read ↗

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