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Article

Molecular Characterization of a Tissue‐Polypeptide‐Specific‐Antigen Epitope and its Relationship to Human Cytokeratin 18

1 Oct 1996

Abstract excerpt

Tissue-polypeptide-specific antigen (TPS) from the human colon adenocarcinoma cell line WiDr was purified using the monoclonal antibody M3 as a probe. Upon SDS/polyacrylamide gradient gel electrophoresis, several TPS-positive bands were detected (corresponding to 13 kDa, 22 kDa and a doublet at 42 kDa). The 13-kDa moiety was purified about 30,000-fold by a 5-step protocol. The electro-phoretically homogeneous...

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Identifiers

PMID: 8917424
DOI: 10.1111/j.1432-1033.1996.00309.x

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