Article
N-terminal quarter part of tetracycline transporter from pACYC184 complements K+ uptake activity in K+ uptake-deficient mutants of Escherichia coli and Vibrio alginolyticus.
Biological & pharmaceutical bulletin - 1 Sept 1995
Nakamura T, Matsuba Y, Ishihara A, Kitagawa T, Suzuki F, Unemoto T
Abstract excerpt
In an attempt to clone a gene encoding the K+ uptake system from Vibrio alginolyticus, two plasmids, pKT2 and pKT4, were derived from pACYC184. These plasmids allowed the growth of K+ uptake-deficient mutant strains of Escherichia coli TK420 and V. alginolyticus FS181 in a low K+ medium. The pKT2...
Topics
- Amino Acid Sequence
- Antiporters
- Bacterial Proteins
- Base Sequence
- Cloning, Molecular
- Escherichia coli
- Molecular Sequence Data
- Mutation
- Plasmids
- Potassium
- Vibrio