N-terminal quarter part of tetracycline transporter from pACYC184 complements K+ uptake activity in K+ uptake-deficient mutants of Escherichia coli and Vibrio alginolyticus.

Abstract excerpt

In an attempt to clone a gene encoding the K+ uptake system from Vibrio alginolyticus, two plasmids, pKT2 and pKT4, were derived from pACYC184. These plasmids allowed the growth of K+ uptake-deficient mutant strains of Escherichia coli TK420 and V. alginolyticus FS181 in a low K+ medium. The pKT2 and pKT4 had an insertion about 7 and 6 kb, respectively, from the genome of V. alginolyticus. We prepared deletion...

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