Article
PCR amplification of DNA from archival specimens. A methodological approach.
Neoplasma - 1 Jan 1996
Pavelić J, Gall-Troselj K, Bosnar M H, Kardum M M, Pavelić K
Abstract excerpt
The experiments were designed to assess whether DNA could be recovered from archival, fixed, paraffin embedded specimens, 1-39 years old, for use in PCR and Southern blot analyses. Specimen fixed in either 10% formalin, Carnoy's or AMeX fixative produced almost equally abundant 318 bp long beta-actin fragment, after 2 x 120 min deparaffinization time, proteinase K DNA extraction and 40, or more, amplification...
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