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Article

Fixation and decalcification of adult zebrafish for histological, immunocytochemical, and genotypic analysis.

BioTechniques - 1 Feb 2002

Moore Jessica L, Aros Michele, Steudel Kimberly G, Cheng Keith C

Abstract excerpt

To facilitate the molecular analysis of tissues in adult zebrafish, we tested eight different fixation and decalcification conditions for the ability to yield DNA suitable for PCR and tissue immunoreactivity, following paraffin embedding and sectioning. Although all conditions resulted in good tissue histology and immunocytochemistry, only two conditions left the DNA intact as seen by PCR. The results indicate...

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PMID: 11848405
DOI: 10.2144/02322st03

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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