Article
Variability in beta-globin and HPV DNA amplification by PCR from fixed tissues.
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc - 1 Sept 1991
Park J S, Leake J F, Sharma B K, Toki T, Kessis T D, Ambros R A, Shah K V
Abstract excerpt
Paraffin-embedded tissues from a variety of sources and treated with different fixatives were tested for beta-globin and HPV amplification by polymerase chain reaction (PCR). In tests of tissues collected in the previous 2 to 3 yr, excellent rates (87% to 93%) for beta-globin amplification were obtained for specimens fixed in buffered formalin, Bouin's, and Hartmann's solutions. In contrast, the rate of...
Topics
- Blotting, Southern
- Cervix Uteri
- DNA, Viral
- Female
- Gene Amplification
- Genetic Variation
- Globins
- Humans
- Papillomaviridae
- Polymerase Chain Reaction
- Vulva