Article
Deletion of the nuclear gene encoding the mitochondrial citrate transport protein from Saccharomyces cerevisiae.
Biochemical and biophysical research communications - 24 Sept 1996
Kaplan R S, Mayor J A, Kakhniashvili D, Gremse D A, Wood D O, Nelson D R
Abstract excerpt
The nuclear gene encoding the mitochondrial citrate transport protein (i.e., CTP1) has been deleted from a haploid yeast strain. The stable yeast deletion strain was constructed by homologous recombination of the HIS3 gene at the CTP1 gene locus. Deletion of the CTP was confirmed by PCR. Immunobl...
Topics
- Carrier Proteins
- Cell Nucleus
- Gene Deletion
- Genes, Fungal
- Genetic Complementation Test
- Haploidy
- Mitochondria
- Molecular Sequence Data
- Open Reading Frames
- Phenotype
- Polymerase Chain Reaction
- Recombination, Genetic
- Reproducibility of Results
- Saccharomyces cerevisiae