Article
Characterization of an OprL null mutant of Pseudomonas putida.
Journal of bacteriology - 1 Oct 1996
Rodríguez-Herva J J, Ramos J L
Abstract excerpt
A Pseudomonas putida oprL null mutant was generated with reverse genetics by using an in vitro-truncated oprL::xylE construct and in vivo allelic exchange. The nature of the mutation introduced in P. putida was confirmed by Southern blotting. Western blots (immunoblots) of peptidoglycan-associate...
Topics
- Bacterial Outer Membrane Proteins
- Catechol 2,3-Dioxygenase
- Cell Membrane
- DNA, Ribosomal
- Dioxygenases
- Escherichia coli Proteins
- Lipoproteins
- Mutagenesis, Insertional
- Mutation
- Oxygenases
- Peptidoglycan
- Phenotype
- Proteoglycans
- Pseudomonas
- Pseudomonas putida
- Recombination, Genetic