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Article

Isolation and characterization of a regulatory gene affecting rhamnolipid biosurfactant synthesis in Pseudomonas aeruginosa.

Journal of bacteriology - 1 Apr 1994

Ochsner U A, Koch A K, Fiechter A, Reiser J

Abstract excerpt

A mutant strain (65E12) of Pseudomonas aeruginosa that is unable to produce rhamnolipid biosurfactants and lacks rhamnosyltransferase activity was genetically complemented by using a P. aeruginosa PG201 wild-type gene library. A single complementing cosmid was isolated on the basis of surface tension measurements of subcultures of the transconjugants by using a sib selection strategy. The subcloning of the...

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Identifiers

PMID: 8144472
DOI: 10.1128/jb.176.7.2044-2054.1994
PMCID: PMC205310

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