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Article

Generation of active [NiFe] hydrogenase in vitro from a nickel-free precursor form.

Biochemistry - 6 Aug 1996

Maier T, Böck A

Abstract excerpt

The maturation process of [NiFe] hydrogenases includes formation of the nickel metallocenter, proteolytic processing of the large subunit, and assembly with the other hydrogenase subunit(s). An in vitro system for the maturation of the large subunit (HycE) of hydrogenase 3 of Escherichia coli leading to an active enzyme was established. The system is based on extracts of an E. coli mutant lacking the...

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PMID: 8756471
DOI: 10.1021/bi960567l

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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Generation of active [NiFe] hydrogenase in vitro from a nickel-free precursor form.PMID 8756471Read ↗

Maturation of the [NiFe] hydrogenases.Semantic proximity Read ↗

Proteolytic cleavage orchestrates cofactor insertion and protein assembly in [NiFe]-hydrogenase biosynthesis.Semantic proximity Read ↗

Analysis of the cleavage site specificity of the endopeptidase involved in the maturation of the large subunit of hydrogenase 3 from Escherichia coli.Semantic proximity Read ↗

Evidence for an oxygen-sensitive iron-sulfur cluster in an immature large subunit species of Escherichia coli [NiFe]-hydrogenase 2.Semantic proximity Read ↗

Strep-tag II affinity purification: an approach to study intermediates of metalloenzyme biosynthesis.Semantic proximity Read ↗

Interaction between hydrogenase maturation factors HypA and HypB is required for [NiFe]-hydrogenase maturation.Semantic proximity Read ↗

Involvement of the GroE chaperonins in the nickel-dependent anaerobic biosynthesis of NiFe-hydrogenases of Escherichia coli.Semantic proximity Read ↗

Analysis of the transcarbamoylation-dehydration reaction catalyzed by the hydrogenase maturation proteins HypF and HypE.Semantic proximity Read ↗

Involvement of hyp gene products in maturation of the H(2)-sensing [NiFe] hydrogenase of Ralstonia eutropha.Semantic proximity Read ↗

[FeFe]- and [NiFe]-hydrogenase diversity, mechanism, and maturation.Semantic proximity Read ↗

SlyD-dependent nickel delivery limits maturation of [NiFe]-hydrogenases in late-stationary phase Escherichia coli cells.Semantic proximity Read ↗

Metal binding activity of the Escherichia coli hydrogenase maturation factor HypB.Semantic proximity Read ↗

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