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Article

Evidence for an oxygen-sensitive iron-sulfur cluster in an immature large subunit species of Escherichia coli [NiFe]-hydrogenase 2.

Biochemical and biophysical research communications - 20 Jul 2012

Soboh Basem, Kuhns Martin, Braussemann Mario, Waclawek Mandy, Muhr Enrico, Pierik Antonio J, Sawers R Gary

Abstract excerpt

Endoprotease-specific C-terminal processing is required to complete the maturation of the large subunit of [NiFe]-hydrogenases. This happens only after synthesis and insertion of the NiFe(CN)(2)CO cofactor by the Hyp maturases has occurred. It is assumed that in the absence of maturation the unprocessed species of the large subunit lacks cofactors. In this study we isolated a variant of the hydrogenase 2 large...

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PMID: 22735263
DOI: 10.1016/j.bbrc.2012.06.096

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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