Article
Mutational screening of APP gene in patients with early-onset Alzheimer disease utilizing mismatched PCR-RFLP.
Clinical genetics - 1 Mar 1996
Nishiwaki Y, Kamino K, Yoshiiwa A, Nagano K, Takeda M, Tanabe H, Nishimura T, Kobayashi T, Yamamoto H, Nonomura Y, Yoneda H, Sakai T, Imagawa M, Miki T, Ogihara T
Abstract excerpt
To elucidate the frequency of mutations of the beta/A4 amyloid protein precursor (APP) gene in early-onset Alzheimer disease, we designed a mismatched PCR-RFLP that can identify all kinds of missense mutations at codon 717 in addition to the seven kinds of known mutations at exon 17. When we scre...
Topics
- Adult
- Alzheimer Disease
- Amyloid beta-Protein Precursor
- Base Sequence
- DNA Primers
- Exons
- Humans
- Japan
- Middle Aged
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length