Article
HLA-B40, B18, B27, and B37 allele discrimination using group-specific amplification and SSCP method.
Human immunology - 1 Apr 1996
Bannai M, Tokunaga K, Lin L, Ogawa A, Fujisawa K, Juji T
Abstract excerpt
We developed a system for discriminating HLA-B40, B18, B27, and B37 alleles using a two-step PCR method followed by SSCP analysis. Fragments (0.8 kb) including exon 2, intron 2, and exon 3 were amplified in the first PCR. We used two sets of primers, one specific for HLA-B60-related alleles and the other specific for HLA-B61-related, B18, B27, and B37 alleles. No amplifications of other class I genes or...
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