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Article

Site-directed tryptophan fluorescence reveals two essential conformational changes in the Na+/H+ antiporter NhaA.

Proceedings of the National Academy of Sciences of the United States of America - 20 Sept 2011

Kozachkov Lena, Padan Etana

Abstract excerpt

NhaA, a Na(+)/H(+) antiporter critical for pH and Na(+) homeostasis in Escherichia coli, as well as other enterobacteria and possibly Homo sapiens, was modified for fluorescence spectroscopy by constructing a functional Trp-less NhaA mutant. Purified Trp-less NhaA lacks the Trp fluorescence emission characteristic of the wild type, thereby providing a background for studying structure-function relationships in...

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Identifiers

PMID: 21873214
DOI: 10.1073/pnas.1109256108
PMCID: PMC3179058

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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