Article
Amino acid conservation in animal glucokinases. Identification of residues implicated in the interaction with the regulatory protein.
The Journal of biological chemistry - 15 Mar 1996
Veiga-da-Cunha M, Courtois S, Michel A, Gosselain E, Van Schaftingen E
Abstract excerpt
To delineate the regions of liver glucokinase that are involved in the binding of its regulatory protein and have therefore been conserved throughout evolution, we have cloned the cDNA of the Xenopus laevis enzyme. It contains an open reading frame of 1374 nucleotides and encodes a protein of 458...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Binding Sites
- Biological Evolution
- Conserved Sequence
- DNA Primers
- DNA, Complementary
- Glucokinase
- Hexokinase
- Humans
- In Vitro Techniques
- Liver
- Models, Molecular
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Rats