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Article

Characterization of guanine and hypoxanthine phosphoribosyltransferases in Methanococcus voltae.

Journal of bacteriology - 1 May 1996

Bowen T L, Lin W C, Whitman W B

Abstract excerpt

Phosphoribosyltransferase (PRTase) and nucleoside phosphorylase (NPase) activities were detected by radiometric methods in extracts of Methanococcus voltae. Guanine PRTase activity was present at 2.7 nmol min(-1) mg of protein(-1) and had an apparent Km for guanine of 0.2 mM and a pH optimum of 9. The activity was inhibited 50% by 0.3 mM GMP. IMP and AMP were not inhibitory at concentrations up to 0.6 mM....

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Identifiers

PMID: 8626317
DOI: 10.1128/jb.178.9.2521-2526.1996
PMCID: PMC177974

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Source receipt: pubmed, retrieved 7 Sept 2026.

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