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Article

Improved adenovirus packaging cell lines to support the growth of replication-defective gene-delivery vectors.

Proceedings of the National Academy of Sciences of the United States of America - 16 Apr 1996

Amalfitano A, Begy C R, Chamberlain J S

Abstract excerpt

Adenovirus (Ad) vectors have been extensively used to deliver recombinant genes to a great variety of cell types in vitro and in vivo. Ad-based vectors are available that replace the Ad early region 1 (E1) with recombinant foreign genes. The resultant E1-deleted vectors can then be propagated on 293 cells, a human embryonal kidney cell line that constitutively expresses the E1 genes. Unfortunately, infection of...

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Identifiers

PMID: 8622940
DOI: 10.1073/pnas.93.8.3352
PMCID: PMC39611

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