Article
Improved adenovirus packaging cell lines to support the growth of replication-defective gene-delivery vectors.
Proceedings of the National Academy of Sciences of the United States of America - 16 Apr 1996
Amalfitano A, Begy C R, Chamberlain J S
Abstract excerpt
Adenovirus (Ad) vectors have been extensively used to deliver recombinant genes to a great variety of cell types in vitro and in vivo. Ad-based vectors are available that replace the Ad early region 1 (E1) with recombinant foreign genes. The resultant E1-deleted vectors can then be propagated on...
Topics
- Adenovirus E1 Proteins
- Adenoviruses, Human
- Base Sequence
- Cell Line
- DNA Primers
- DNA, Viral
- Defective Viruses
- Gene Deletion
- Genes, Viral
- Genes, pol
- Genetic Complementation Test
- Genetic Vectors
- Humans
- Molecular Sequence Data
- Mutation
- RNA, Viral
- Temperature
- Transfection