Article
Isolation and characterization of the recA gene of Bordetella pertussis.
Molecular microbiology - 1 Jul 1990
Kuhl S A, McCreary R P, Bannan J D, Friedman R L
Abstract excerpt
This report describes the detection and cloning of the Bordetella pertussis recA gene. Escherichia coli clones having recombinant plasmids containing the B. pertussis recA gene were isolated by complementing an E. coli RecA- mutant's inability to survive in the presence of methylmethanesulphonate (MMS). This gene was shown to complement the deficiency of E. coli RecA- strains to tolerate the DNA-damaging effects...
Topics
- Blotting, Southern
- Bordetella pertussis
- Cloning, Molecular
- DNA Damage
- DNA, Bacterial
- DNA-Directed DNA Polymerase
- Drug Resistance, Microbial
- Genes, Bacterial
- Methyl Methanesulfonate
- Mutation
- Plasmids